Fig. 2
From: Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
Reduced editing activity of high-fidelity Cas9 variants at target sites with a non-G 5’ nucleotide. a Indel frequencies of Cas9-WT and Cas9 variants at 26 endogenous target sites with an HX19 sequence obtained using gX19 sgRNAs in HeLa cells (H: not G [C or T or A]). The sequences and indel frequencies (%) of the 26 target sites are described in Additional file 1: Table S1. * P < 0.05, ** P < 0.01, **** P < 0.0001. b Distributions of relative indel frequencies of eCas9-1.1 and Cas9-HF1 normalized to that of Cas9-WT. Means and medians of relative indel frequencies are represented in Additional file 1: Table S2. Box and whisker plots: center lines show the medians; crosses show the means. c Indel frequencies (measured by targeted deep sequencing) at six on-target sites with a 5’ C or 5’ T nucleotide obtained using gX19 and gX20 sgRNAs in HeLa cells. PAM sequences are shown in blue. Error bars, s.e.m. of three biological replicates