Fig. 10

Mechanism of CRISPR/Cas9-mediated targeted chromosome elimination. a Results of DNA-FISH analysis on the gene-edited ES cells. Percentages of cells (including dividing cell, Y:X = 2:2 or 0:2) exhibiting different genotype ratios. n is the sample size of counted cells. b Sex ratio of mice generated by gene editing. n is the sample size of mice generated by gene editing. c Results of DNA-FISH analysis of the ratio of blastomeres with Y deletion in each male embryo with Ssty2 targeting at different stages. Data are presented as means ± SEM (n = 4 to 12; ***P < 0.001, **P < 0.01, *P < 0.05, t test). d Results of DNA-FISH analysis on cells treated with KU-55933 or not. Percentages of cells (including dividing cells, Y:X = 2:2 or 0:2) exhibiting different genotype ratios. n is the sample size of counted cells. e Examples of Y chromosome exclusion from nucleus after gene editing. DNA-FISH analysis of mouse ES cells or two-cell embryos with isolated Y chromosome following Ssty2 targeting. Green, Y; red, X; blue, DNA; N, primary nucleus; Arrows: green arrows, Y; red arrows, X. Bar, 20 μm