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Fig. 1 | Genome Biology

Fig. 1

From: Functional assessment of human enhancer activities using whole-genome STARR-sequencing

Fig. 1

Experimental approach and validation of WHG-STARR-seq. a Schematic representation showing experimental setup and approach for WHG-STARR-seq. b Genomic snapshot displaying the GPI locus region as detected by WHG-STARR-seq. There is a strong enhancer region approximately 10 kb upstream of GPI transcriptional start site and another weaker enhancer region in the 3’UTR of GPI. Each blue track signifies normalized WHG-STARR-seq signal of each biological replicate. The gray track represents normalized WHG-STARR-seq signal of input library. c Distribution of WHG-STARR-seq enhancer activity of all detected enhancers. WHG-STARR-seq shows a wide dynamic range of enhancer signal normalized to INPUT (1.33–119.12, median = 3.08). d Distribution of number of detected enhancers associated per gene (enhancers are assigned to their nearest genes). e The enhancer activity of six active (red) and nine inactive (blue) enhancers were validated using traditional luciferase assays in biological triplicates. A strong correlation was observed between luciferase signal and WHG-STARR-seq enhancer activity

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