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Fig. 4 | Genome Biology

Fig. 4

From: Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7

Fig. 4

Validation of candidate iCLIP and RIP-seq targets by RIP-qPCR. RIP-qPCR analysis of iCLIP and RIP targets that are circadianly regulated (AtGRP7, DRM2, HSPRO2, PSBP-1, AT3G15450, AILP1, FER1, and FAD2) or cold regulated (COR15A, COR15B, COR413-PM1, and KIN1), iCLIP-only targets (CCL and PR4), and RIP-only targets (ERF4, WRKY33, and STZ) in AtGRP7::AtGRP7-GFP grp7-1 and AtGRP7::GFP-only plants. Transcript levels in the GFP Trap precipitate (IP+) or RFP Trap precipitate (IP−) are presented relative to the transcript levels in the input. Data represent mean ± standard deviation of three biological replicates. PP2A and TGH as unbound transcripts serve as negative controls

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Genome Biology

ISSN: 1474-760X

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