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Fig. 4 | Genome Biology

Fig. 4

From: The human lncRNA LINC-PINT inhibits tumor cell invasion through a highly conserved sequence element

Fig. 4

A highly conserved short region of LINC-PINT is required for its function. a Schematic representation of alignment signatures found for mouse Lincpint and the orthologus human LINC-PINT using slncky Evolution Browser [10]; the conserved region between mouse and human is emphasized and the conserved sequences between mammals [12] are underlined in red (CE1) and blue (CE2). Conservation values of BLAST are summarized in the bottom table. b Schematic representation of FL LINC-PINT clone and the LINC-PINT mutants; HCR, LCR, CE1 deletion (ΔCE1), CE2 deletion (ΔCE2), and CE1-2 deletion (ΔCE1-2) (left), and the invasion phenotype observed upon their expression. c, d Invasion assay performed as in Fig. 2d in HCT116 cells expressing the indicated forms of LINC-PINT or an empty vector (CTRL). e Schematic of LINC-PINT fragment deletion by CRISPR-Cas9. f Invasion phenotype of HCT116 cellular clones with homozygous deletion of CE1 sequence (CL25, CL124, CL143, and CL249) or normal cells (WT pool and WT28). Significance was determined by Mann–Whitney U test (*P < 0.05, **P < 0.01)

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