Fig. 3
From: The human lncRNA LINC-PINT inhibits tumor cell invasion through a highly conserved sequence element
Enforced expression of LINC-PINT decreases metastasis initiation of CRC cells. a–d Intrasplenic mice inoculation with 2.5 × 105 HCT116 control cells (CTRL) (n = 6) or LINC-PINT overexpressing HCT116 cells (LINC-PINT) (n = 6). a Schematic representation of liver metastasis mice model induced by intrasplenic injection of colon cancer cells. b Representative pictures of liver metastases (blue arrows) at time of sacrifice (left), hematoxylin and eosin (H&E)-stained sections scanned on an Aperio Scan Scope AT (middle) and 4X magnifications of H&E slides; healthy and tumoral tissue is pointed out with (H) and (T), respectively. c Quantification of number of liver metastasis, micrometastases ≤ 2 mm and macrometastases ≥ 2 mm. d Graphic representation of percentage of tumoral area per mice liver quantified on Aperio Image Scope (Leica Biosystems, Buffalo Grove, IL, USA) (*P < 0.05, P < 0.01 two-tailed Student’s t-test)