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Fig. 2 | Genome Biology

Fig. 2

From: The human lncRNA LINC-PINT inhibits tumor cell invasion through a highly conserved sequence element

Fig. 2

LINC-PINT overexpression inhibits the migration and invasion capability of lung and colon cancer cells. a Xenograft growth in nude mice injected with HCT116 CTRL cells (n = 6) or LINC-PINT overexpressing HCT116 cells (n = 6). Mean and standard deviation is shown. *P value < 0.05 determined by Student’s t-test. b Relative numbers at indicated times of control cells (transduced with empty vector) or cells expressing LINC-PINT. c Wound healing assay of control and LINC-PINT overexpressing HCT116 cells. Representative image of the invaded area as captured 12 h after the scratch (black lines depict the invasive front at 0 and 12 h, respectively) (upper panel). Medium plots represent single-cell tracks taken every 5 min for 12 h to ten different cells. d Invasion capacity of 105 HCT116, A549, DLD1 CTRL cells and their equivalent LINC-PINT overexpressing cells analyzed using transwell chambers coated with Matrigel at 36 h. The number of invading cells is counted from images of five random fields per transwell. Data are shown as mean ± SD of the fold change of invading cells relative to control cell line of three independent biological replicates. e Cell transmigration across collagen-coated membranes. Control HCT116 and LINC-PINT overexpressing cells were allowed to migrate across collagen-coated wells for 24 h. Total number of cells in the lower side of the membrane was counted on images taken from five random fields per transwell. Data are represented as mean ± SD of migrating cells from three independent biological replicates. f, g HCT116 and A549 LINC-PINT cells were transfected with two independent antisense oligos (ASO) to knockdown LINC-PINT (ASO h5 and ASO h7), or a control ASO, and their invasion capacity was quantified as in (d)

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