Fig. 1

Characterization of antisense transcripts. a Overview of RNA-sequencing data used in this study. In addition to published data, we generated RNA-sequencing data from poly(A)-selected total RNA from HSV-1-infected WI-38 cells. b Clustering of antisense transcripts. Antisense transcripts were clustered based on the fold change between pulse labeling sequencing data at different timepoints after infection compared to mock-infected cells. c Clustering of correlations of antisense transcript expression values between timepoints and biological replicates. d Examples of antisense transcripts, from top to bottom: BBC3 antisense (internal), RFX1 antisense (convergent), divergent SLC27A4as. Coverage profiles for poly(A)-selected (WI-38 cells) and Ribozero treated (human foreskin fibroblast (HFF) cells) total RNA-sequencing data are shown. Sense genes are depicted in orange running left to right, antisense transcripts in cyan running right to left. Respective transcription starts and chromosome regions are indicated. Refseq annotations are shown in dark blue, locations of RT-qPCR primer pairs in red