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Fig. 1 | Genome Biology

Fig. 1

From: Editing inducer elements increases A-to-I editing efficiency in the mammalian transcriptome

Fig. 1

Structural requirements for efficient editing at the Q/R site of the GluA2 transcript. a GluA2 RNA structure at exon11–intron11. Exonic sequence is illustrated in blue and intronic sequence in black. The Q/R site is located in exon 11 and indicated with a red dot. The region in grey illustrates the position of the EIE, 45 nt downstream of the Q/R site. b Left: the wild-type construct, GA2Q/R containing the Q/R editing site and the EIE; the GA2Q/R-ΔEIE mutant where the EIE has been deleted; the GA2Q/R-US EIE where the EIE has been moved to a position 50 nt upstream of the Q/R site; and GA2Q/R-US G3 EIE where the Gabra-3 EIE is placed 50 nt upstream of the Q/R site. Right: sequencing chromatograms illustrating editing of the different GluA2 reporters by endogenous ADAR2 in HeLa cells. c Quantification of editing efficiency at the Q/R site from the different GA2Q/R constructs in HeLa cells. The mean value of the ratio between the A and G peak heights from three individual experiments is calculated as the percentage of editing. Error bars are standard deviation

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