Fig. 9

ERβ association with RISC and a subset of small and long RNAs in BC cell cytoplasm. a Association of ERβ with the RISC proteins AGO2, DICER, and TRBP2 analyzed by western blotting following immunoprecipitation of cytosolic extracts with IgG (TAP) in Ct-ERβ and, as control, wild-type (wt; ERβ−) MCF-7 cells. b The enrichment coefficient of all the RNAs (>4500) co-immunoprecipitated with ERβ. Blue dots mark the 878 RNAs selected based on the enrichment factor in ERβ + samples and the relative enrichment (ratio between enrichment factors in ERβ + and ERβ−, used as negative control). c Direct comparison of RIP-Seq results relative to the 878 ERβ-interacting RNAs in ERβ + with respect to ERβ − samples. d The relative abundance of different classes of small (right) and long (left) RNAs, respectively, found associated with ERβ by RIP-Seq