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Fig. 5 | Genome Biology

Fig. 5

From: The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading

Fig. 5

Characterization of ERβ–AGO2 interaction. a Co-immunoprecipitation of ERβ and AGO2 from cytosolic extracts of Ct-ERβ or wild-type (wt; ERβ−, control) cells transiently transfected with a Myc-tagged AGO2 expression vector. b Upper panel: RNA evaluation before and at different times after treatment with RNAse A on either nuclear or cytosolic extracts. Lower panel: co-immunoprecipitation of ERβ with AGO2 from nuclear (NUC) and cytoplasmatic (CYT) extracts before and at different times after RNAse A treatment

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Genome Biology

ISSN: 1474-760X

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