Fig. 1

Effects of unliganded ERβ on the BC cell transcriptome and alternative RNA splicing. a The fraction of differentially expressed genes detected in both Ct-ERβ- and Nt-ERβ-expressing cells (red/green) or in only one of the two cell lines (white). b Functional annotation by Ingenuity Comparative Analysis on genes differentially expressed in BC cells expressing either Ct-ERβ or Nt-ERβ (fold change cut-off |1.5|, false discovery rate (FDR) ≤ 0.05). The red line indicates the p value threshold. c Alternative splicing events occurring in the two ERβ-expressing cell lines. Inclusion and exclusion behaviors for each event are shown (FDR ≤ 0.05; inclusion/exclusion cut-off |0.1|). d Heatmap showing differentially expressed transcripts subject to alternative splicing events in both ERβ-expressing cell lines