Fig. 4
From: Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
Cleavability ranking of the targets by nuclease variant as well as fidelity ranking (eSpCas9 < SpCas9-HF1 < HeFSpCas9) of the nucleases on these targets is apparent. Disruption and indel formation activities of SpCas9 nucleases programmed with perfectly matching or partially mismatching sgRNAs. a Heat maps showing the relative activities (white to green) of the nuclease variants compared to the WT for each of the targets and the ratios of off-target to on-target disruption activities (blue to white) of the WT and mutant nucleases measured employing the indicated target and mismatching spacer sequences; grey and black boxes indicate not determined due to diminished on-target activities and sample loss, respectively. b–d Specificities (on-target:off-target ratio) of the nucleases assessed by b disruption activities, c deep-sequencing on indel formation (eSpCas9 and SpCas9-HF1) and disruption activities (SpCas9), and d deep-sequencing on indel formation (HeFSpCas9) and disruption activities (SpCas9, eSpCas9, SpCas9-HF1)