Fig. 1

Example of erroneous genome arithmetic operations because of integrating data from incompatible genome builds. The sequence intervals in A.bed (hg19) correspond to the intervals in B.bed (hg38). However, when genome arithmetic operations (like intersection) are performed in the absence of genome build information, there will be no overlap between the intervals in both the files, as the coordinates appear non-overlapping. If it is known that the files correspond to two different genome builds, lift-over of coordinates in one file would result in the detection of overlapping genomic intervals between both the files