Fig. 5

Examples of complex Splat simulations. a A principle components analysis (PCA) plot of a simulation with six groups with varying numbers of cells and levels of differential expression. b A PCA plot of a simulation with two groups (pink and blue) and two batches (circle and triangle). PC1 separates groups (wanted biological variation) while PC2 separates batches (unwanted technical variation). c A PCA plot of a simulation with differentiation paths; the colored gradient indicates how far along a path each cell is from blue to pink. A progenitor cell type (blue circles) differentiates into an intermediate cell type (pink circles/blue triangles or diamonds), which becomes one of two (pink triangle or diamond) mature cell types