Fig. 4

Normal organ histology in the absence of ADAR1 RNA editing. a Hematoxylin and eosin (H&E)-stained sections of the indicated organs of Adar1 ^E861A/E861A Ifih1 ^-/- (n = 4; 2 male, 2 female) and Adar1 ^E861A/+ Ifih1 ^-/- (n = 4; 2 male, 2 female) control littermates. 10× magnification with 100-μM scale bar; 40× inset for each. b–g MicroCT analysis of tibial bone; C57BL/6 (n = 4), Adar1 ^+/+ Ifih1 ^-/- (n = 4), Adar1 ^E861A/+ Ifih1 ^-/- (n = 7), Adar1 ^E861A/E861A Ifih1 ^-/- (n = 9). b Representative images of reconstructed trabecular region of the secondary spongiosa within the proximal tibia with color-coded quantitative mineralization from indicated genotypes. c Tibial length, (d) Trabecular bone volume, (e) trabecular number, (f) trabecular separation, and (g) trabecular thickness. h–k MicroCT analysis of cortical bone of the same samples used for panels (b–g). h Representative images of reconstructed cortical bone with color-coded quantitative mineralization, (i) cortical thickness, (j) cortical bone area, and (k) endocortical perimeter. Results are mean ± SEM; significance tested using ANOVA, *P < 0.05, **P < 0.01