Fig. 1

New methods for single-cell protein profiling. In the antibody barcoding with photocleavable DNA platform (ABCD) platform, cells are permeabilized and stained using a panel of antibodies. The tagged DNA is cleaved, amplified by PCR, and sequenced using Nanostring technology. The Abseq method is performed by encapsulating stained cells, tagging each cell with a unique barcode, and PCR amplifying and sequencing using Illumina technology. The cellular indexing of transcriptomes and epitopes by sequencing (CITE-Seq) method utilizes poly(A) oligonucleotides to profile cell-surface proteins and it can be coupled with single-cell RNA-seq protocols such as Drop-Seq and Chromium (10×)