Fig. 8

Predicted targets of miRNA candidates in lycophytes and ferns. a Top 20 protein superfamily or domain hits in predicted targets of miRNA candidates. Each color represents one type of protein family or domain. The y-axis shows the numbers of hits in domain searches using all predicted targets of miRNA candidates from lycophytes and ferns. b Nucleotide variations in a group of orthologous genes targeted by miR166 family members. The coding sequences of these genes were aligned using 21-nt sliding windows. The sequences were perfectly aligned at the miR166 binding sites. Nucleotide variations within each window were calculated and plotted. The x-axis positions represent those of the 5′ ends of windows. The red line indicates the average nucleotide variations at each position and the grey area shows the standard errors of averages. The green “miR166” label and dotted lines mark the location of the binding sites of miR166 family members