Fig. 3

Dissection of genetic regulation of the fatty acid content in soybean. a Candidate genes in the lipid metabolic pathway that are responsible for the variation of fatty acid (FA) synthesis in soybean germplasm. The pathway is modified from Arabidopsis. The dotted lines represent multiple reaction steps. b Plot of the total FA content against the accumulation of high-oil-content alleles. The x-axis indicates the number of accumulated high-oil alleles from all candidate genes in the soybean germplasm; the y-axis shows the total FA content in the corresponding population. c Total FA content of the germplasm from low-latitude and high-latitude areas. ***P < 0.001 (one-sided Student’s t-test, n = 461, 219). d Proportion of accumulated high-oil alleles in low-latitude and high-latitude populations. ACP acyl carrier protein, DAG diacylglycerol, G3P glycerol-3-phosphate, FA fatty acid, LPA lysophosphatidic acid, PC phosphatidylcholine, PYR pyruvate, TAG triacylglycerol, ACNA acyl-CoA n-acyltransferase, FAD fatty acid desaturase, FatB fatty acyl-ACP thioesterase B, PDHK pyruvate dehydrogenase kinase, PLC phospholipase C, PLD phospholipase D, ROD1 reduced oleate desaturation 1, SAD stearoyl-acyl-carrier-protein desaturase, ER endoplasmic reticulum