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Fig. 3 | Genome Biology

Fig. 3

From: Large scale genomic reorganization of topological domains at the HoxD locus

Fig. 3

Impact of increasing distance on interactions with Hoxd13. a The large inversions displacing regulatory islands away from Hoxd13. b DNA FISH signals for Hoxd13 (green) and island I (red) in distal limb bud autopod cells dissected from homozygous mutant embryos for either one of two large inversions. Scale: 1 μm. Below are shown the distribution of spatial distances in the wild-type (WT, green) and the inverted (purple) allele. c, Quantification of 3D distance showing the increase in distance observed in b (Mann–Whitney test, p < 0.001). d 4C-seq profile using Hoxd13 as a viewpoint either in control (green) or the 2.4-Mb HoxD Inv(TpSB1-Itga6) inversion (orange). Above are aligned the heatmaps generated using data from [9] with C-DOM indicated by a blue triangle. The Hoxd13 viewpoint is indicated with a red arrowhead. The breakpoints of the inversion are shown by the red arrows. The red star indicates the position of island III where contacts are lost in the inversion. e Close-up of region boxed in d where ectopic contacts are increased in both HoxD Inv(TpSB1-Itga6) and HoxD Inv(Nsi-Itga6) mutant samples

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