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Fig. 5 | Genome Biology

Fig. 5

From: Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding

Fig. 5

Drug-induced activation of the Houba retrotransposon in O. sativa. Mobilome analysis of DNA extracted from seedlings after growth under control conditions (C), A (5 μg/ml), Z (40 μM) or the combination of A&Z. a Logarithmic ratio of the depth of coverage obtained after aligning the sequenced reads on one Houba element. b Primer localization (black bar, Houba element; arrows, PCR primers; red box,LTR). c Circular forms of Houba are specifically detected in plants treated with A&Z using inverse PCR with primers shown in (b). d Specific PCR on chloroplast DNA is shown as a loading control. Total DNA subjected to a rolling circle amplification was used as a template. M indicates the size marker

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