Fig. 4

Transparent testa phenotype of hc line 3 co-segregates with an ONSEN insertion in TT6. Seed phenotypes (a) and corresponding genotypes (c) of a segregating F2 population (lanes 1–22) obtained from a cross between the WT and hc line 3 (hc) are shown. b Primers used for genotyping of the ONSEN insertion. For the WT-PCR depicted in the upper part of c the light (tt6 fw) and dark (tt6 rev) green primers flanking the TT6 locus (AT3G51240) were used. The ONSEN insertion in TT6 was detected by a combination of the light green primer with the red primer specific to the ONSEN LTR (Copia 78 3′ LTR, red arrow). M indicates the size marker. Primer sequences are given in Additional file 1: Table S1