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Fig. 3 | Genome Biology

Fig. 3

From: Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding

Fig. 3

Drug-induced mobilization of ONSEN in WT Arabidopsis plants. a Transposon display testing seedlings in the S2 generation of WT plants for novel ONSEN insertions: lanes a to c show HS-treated plants; lanes 1 to 7 show hc lines 1–7 treated with HS and A (5 μg/ml) and Z (40 μM), M indicates the size marker. b ONSEN copy number in the S1, S2 and S3 generations measured by qPCR (mean ± s.e.m, n = 3 technical replicates, values relative to ACTIN2). c, d Photographs of S2 plants showing both homogeneous and environment-dependent phenotypic variability induced by the ONSEN mobilization when grown under long (c) and short day (d) conditions. qPCR data for the S3 generation of line 6 in b as well as pictures of phenotypes in c and d are missing due to severe infertility and extinction of this line

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