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Fig. 2 | Genome Biology

Fig. 2

From: Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding

Fig. 2

Simultaneous inhibition of DNA methyltransferases and Pol II reduces global CHH methylation and mimics the TE silencing deficiency of the nrpd1 background. a Genome-wide DNA methylation levels in the WT after CS and CS plus treatment with A (5 μg/ml), Z (40 μM), or a combination of A and Z (A&Z) for three sequence contexts (brown for CG, yellow for CHG and blue for CHH). b Same as a but only depicting the CHH context for clarity. c Methylome data of treated and untreated plants at an ONSEN locus located on Chr 1 (ONSEN is indicated in yellow, its LTRs in red). d Northern blot of ONSEN transcripts directly after CS, HS and HS plus treatment with A, Z or a combination of A&Z in the WT and after HS in nrpd1 plants. The black arrow indicates the ONSEN full-length transcript. Below, a Midori-stained agarose gel is shown as a loading control. e ONSEN copy number measured by qPCR directly after CS and HS treatments in WT, rdr6, dcl2/3/4 and nrpd1 seedlings directly after CS, HS and HS plus treatment with A, Z or a combination of A&Z (mean ± s.e.m, n = 3 biological repetitions, values relative to ACTIN2; *P < 0.05, **P < 0.01)

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