Fig. 2From: Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breedingSimultaneous inhibition of DNA methyltransferases and Pol II reduces global CHH methylation and mimics the TE silencing deficiency of the nrpd1 background. a Genome-wide DNA methylation levels in the WT after CS and CS plus treatment with A (5 μg/ml), Z (40 μM), or a combination of A and Z (A&Z) for three sequence contexts (brown for CG, yellow for CHG and blue for CHH). b Same as a but only depicting the CHH context for clarity. c Methylome data of treated and untreated plants at an ONSEN locus located on Chr 1 (ONSEN is indicated in yellow, its LTRs in red). d Northern blot of ONSEN transcripts directly after CS, HS and HS plus treatment with A, Z or a combination of A&Z in the WT and after HS in nrpd1 plants. The black arrow indicates the ONSEN full-length transcript. Below, a Midori-stained agarose gel is shown as a loading control. e ONSEN copy number measured by qPCR directly after CS and HS treatments in WT, rdr6, dcl2/3/4 and nrpd1 seedlings directly after CS, HS and HS plus treatment with A, Z or a combination of A&Z (mean ± s.e.m, n = 3 biological repetitions, values relative to ACTIN2; *P < 0.05, **P < 0.01)Back to article page