Fig. 1

Pol II represses the HS-dependent mobility of the ONSEN retrotransposon in Arabidopsis. ONSEN copy number in Arabidopsis seedlings measured by qPCR directly after CS and HS treatments. a In the WT and the nrpb2-3 mutant and after HS plus treatments with α-amanitin (A; 5 μg/ml) or zebularine (Z; 10 μM) (mean ± standard error of the mean (s.e.m.), n = 6 biological repetitions). b In the WT and after HS plus treatment with A at different concentrations (μg/ml) as specified on the x-axis (mean ± s.e.m., n = 4 biological repetitions). c In the WT and after HS plus treatment with Z (40 μM) or a combination of A (5 μg/ml) and Z (A&40Z) (mean ± s.e.m., n = 3 biological repetitions). d In the WT after chemical treatment with A (5 μg/ml), Z (40 μM), a combination of A and Z (A&Z) or in the nrpb2-3 and nrpd1 backgrounds following CS (mean ± s.e.m., n = 3 biological repetitions). All values are relative to ACTIN2. *P < 0.05, **P < 0.01