Fig. 7
5-aza-2′-deoxycytidine affects nucleosome occupancy. a Liquid chromatography–mass spectrometry analysis showing loss of DNA methylation after treatment of HCT116 cells with 5 μM 5-aza-2′-deoxycytidine for 2 and 6 days. b Volcano plot showing the distribution of DMRs identified after 6 days of treatment. Blue dots represent hypomethylation (n = 15,761) and red dots represent hypermethylation (n = 173). c Density plots showing the distribution of number of CpGs in the DMRs and the width of DMRs identified in the study. d A Venn diagram showing the overlap between the OCSs identified with and without 6 days of 5 μM 5-aza-2′-deoxycytidine treatment. e The log2 fold change of OCS peaks in different genomic regions for samples treated with and without 6 days of 5 μM 5-aza-2′-deoxycytidine treatment. f The log2 fold change of DMRs in different genomic regions of samples treated with and without 6 days of 5 μM 5-aza-2′-deoxycytidine treatment