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Fig. 6 | Genome Biology

Fig. 6

From: The Arabidopsis SWI/SNF protein BAF60 mediates seedling growth control by modulating DNA accessibility

Fig. 6

BAF60 and PIF4 actively compete in vivo for their targets. Seven-day-old plantlets of the two BAF60 RNAi lines, a BAF60 OE line, and the corresponding WT (WS and Col0, respectively) were used to analyze PIF4 binding on selected target genes (IAA19, ST2A, and XTR7) by ChIP-qPCR. PIF4 binding to targets is increased in the darkness in the BAF60 RNAi lines in comparison with the WT. On the contrary, its binding was diminished in the BAF60 OE line, indicating that BAF60 and PIF4 compete for their targets and that changes in BAF60 levels affect PIF4 binding. Data are average of three technical replicates ± standard deviation. Asterisks indicate significantly different values (Student’s t-test, P < 0.05). D dark, L light

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