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Table 1 Genomic lesion and mRNA splicing results of single cell clones

From: CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Gene/clone sgRNA target Allele Genomic lesion Exon inclusion (%)
Kras (KP1) Exon 2 1 -CG 64.0 ± 9.1b
  2 -C
Krasa (KP2) Exon 2 1 -GG 68.0 ± 7.1b
Ctnnb1c Exon 3 1 -CCA 100
  2 832 bp deletion New mRNA isform with part of intron 2 and exon 4
p65 clone 15 Exon 6 1 +A 100
  2 2.2 kb deletion (exons 5, 6, 7) ND
p65 clone 31a Exon 6 1 +A 100
  1. aClones with one allele
  2. b% exon inclusion is mean ± s.d. (n = 3 for KP1 and n = 2 for KP2)
  3. cClone in Additional file 1: Figure S6
  4. ND not determined