Table 1 Genomic lesion and mRNA splicing results of single cell clones
From: CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Gene/clone | sgRNA target | Allele | Genomic lesion | Exon inclusion (%) |
|---|---|---|---|---|
Kras (KP1) | Exon 2 | 1 | -CG | 64.0 ± 9.1b |
2 | -C | |||
Krasa (KP2) | Exon 2 | 1 | -GG | 68.0 ± 7.1b |
Ctnnb1c | Exon 3 | 1 | -CCA | 100 |
2 | 832 bp deletion | New mRNA isform with part of intron 2 and exon 4 | ||
p65 clone 15 | Exon 6 | 1 | +A | 100 |
2 | 2.2 kb deletion (exons 5, 6, 7) | ND | ||
p65 clone 31a | Exon 6 | 1 | +A | 100 |