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Fig. 3 | Genome Biology

Fig. 3

From: CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Fig. 3

Cas9 nuclease activity required for skipping of one or more exons. a RT-PCR analysis of Ctnnb1 mRNA in KP cells transduced with lentiviruses that encode sgCtnnb1.2 and nuclease-defective Cas9 (dCas9), dCas9-KRAB fusion, or WT Cas9. RT-PCR was performed using primers in exons 2 and 7 on transduced KP cell populations after puromycin selection and FACS sorting. The exon length and reading frame phase are shown. Only the exon 2-4 splice product retains an in-frame β-Catenin coding sequence. b RT-PCR analysis of Ctnnb1 mRNA in KP cells transduced with lentiviruses that encode Cas9 and sgGFP, sg3, or sg5. “–”, untreated

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