Fig. 4

Temperature induced-flowering is compromised in mutant of H3K36me3 writers, eraser, and readers. a Cartoon illustrates regulation of H3K36me3 and splicing according to the adaptor-complex model in Arabidopsis. H3K36me3 modified histones are bound by an adapter, such as MRG1 and MRG2, that interacts with splicing regulators (SRs), such as PTB. The splicing regulators act in promoting or preventing AS. b Flowering time of WT, sdg8-2, and sdg26-1 plants growing at 16 °C constantly or moved to 25 °C after five weeks. In the top histogram, flowering time is expressed as numbers of DTB. In the bottom histogram, the ratio of DTB between the two conditions is shown. In plants with low levels of H3K36me3, flowering is not promoted by higher temperature. ** indicates significant differences at p value < 0.001 while * indicates p value < 0.05 according to the Student’s t-test. c Flowering time of WT, jmj30-1, and JMJ30-OE plants is shown as in (b). Loss of JMJ30 increases the response to temperature-induced flowering, while an increase in JMJ30 activity causes an opposite effect. d Flowering time of WT, mrg1-1 mrg2-3 plants are shown as in (b). mrg1-1 mrg2-3 plants are less responsive to temperature