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Fig. 6 | Genome Biology

Fig. 6

From: The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci

Fig. 6

The effects of PKL on the silencing of genes and TEs. a Heatmap showing the relative transcript levels of the 50 derepressed genes/TEs in pkl that overlapped with hypo DMRs. b Overlaps among derepressed genes/TEs identified in pkl, nrpd1, and nrpe1 that overlapped with their respective hypo DMRs. c qRT-PCR verification of 12 upregulated transposable elements identified in pkl. d qRT-PCR verification of five upregulated genes identified in pkl. Transcript levels relative to WT were shown. Error bars represent standard deviations of three biological replicates. e Boxplots of the mRNA and DNA methylation levels of the 42 genes/TEs that are derepressed in pkl but not in RdDM mutants as shown in (b). f Boxplots of the mRNA and DNA methylation levels at the promoter region of the 50 genes/TEs that are derepressed in both nrpd1 and nrpe1 but not in pkl as shown in (b)

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Genome Biology

ISSN: 1474-760X

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