Skip to main content
Fig. 6 | Genome Biology

Fig. 6

From: Regulatory landscape fusion in rhabdomyosarcoma through interactions between the PAX3 promoter and FOXO1 regulatory elements

Fig. 6

Virtual-HiC of the PAX3-FOXO1 locus in RMS cells predicts the generation of a new TAD. a 4C-seq profiles using nine different viewpoints (VP1–VP9) spanning 3.5 Mb. The locations of the viewpoints are indicated above the graph. The location of the fusion PAX3:FOXO1 gene and other coding sequences is indicated, as well as the position of the RMS breakpoint. Green and orange boxes indicate reads mapped to Chr2 or Chr13, respectively. b The virtual-Hi-C generated from the 4C-seq data was subjected to a D.I. analysis to determine the location of TAD borders. The upstream and downstream borders thus defined closely match those obtained by D.I. analyses of human Hi-C data while a novel TAD encompassing the PAX3-FOXO1 fusion locus is predicted. The positions of the viewpoints (pale green circles), the promoters of the genes in the region (coloured hexagons) and the borders identified by D.I. analysis (coloured boxes) are indicated. The chromosomal coordinates of the predicted borders are provided underneath. 3D chromatin architecture model for the locus encompassing the translocation in ARMS, (c) showing the contribution of both chromosome regions to the predicted new TAD and (d) the location of promoter sequences within the TAD

Back to article page