Fig. 4

Crossing the TAD border can drive ectopic transgene expression from enhancers in the adjacent domain. a Detail of the Hi-C data from mouse ES cells at the TAD border (green box). H3K27ac marks in different mouse tissues are shown underneath, as well as the position of the two coding genes in the region and the relative positions of the three BAC clones used in the study. The 5' ends of the clones cross the TAD border, although in the case of B38, its end maps within the border region. There are strong active-enhancer marks in the non-overlapping region between B116 and B61. b Expression patterns of Foxo1 ^Gt-β-GEO/+, B38Z, B61Z and B116Z at 9.5 dpc. B116Z drives strong expression in the CNS, while B61Z does not; B38Z drives strong vascular expression like the gene-trap allele. D.I. directionality index