Fig. 2

FoxO1 and Pax3 promoter interactions are restricted to their respective TADs. (a) FOXO1/FoxO1 and (b) PAX3/Pax3 Hi-C data (2, 5; 20 kb resolution) coincide with the interactions detected by 4C-seq. The size and position of the FOXO1 and FoxO1 TADs, as determined by D.I. analyses, are very similar despite the ancestral recombinations that occurred immediately upstream of the genes. Names and positions of the genes in the different loci are indicated. The transcriptional direction of the different genes is indicated. The percentage of accumulated reads 1 Mb at either side of the FoxO1 and Pax3 promoters and within the TADs are shown. In the human genome, the positions of the calculated borders for (a) the FOXO1 locus are (left to right) Chr13: 41,902,000; Chr13: 41,362,000; Chr13: 40,262,000 and Chr13: 39,502,000 and for (b) the PAX3 locus are (left to right) Chr2: 223,817,756; Chr2: 223,511,756; Chr2: 223,171,756 (sub-TAD boundary); Chr2: 222,871,756 and Chr2: 222,451,756