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Fig. 1 | Genome Biology

Fig. 1

From: Optimizing complex phenotypes through model-guided multiplex genome engineering

Fig. 1

Workflow for improving phenotypes through model-guided multiplex genome editing. First, an initial set of target alleles (hundreds to thousands) is chosen for testing based on starting hypotheses. These targets may be designed based on differences from a reference strain, synthesis or design errors, or biophysical modeling. Multiplex genome editing creates a set of modified clones enriched with combinations of the targeted changes. Clones are screened for genotype and phenotype and predictive modeling is used to quantify allele effects. The workflow is repeated to validate and test new alleles. Beneficial alleles are combined to create an optimized genotype

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