Fig. 2
From: PRC2 represses transcribed genes on the imprinted inactive X chromosome in mice
RNA FISH analysis of X-linked gene expression in Eed –/– TSCs. Left, RNA FISH detection of Xist RNA coating (green) and RNAs of four X-linked gene (Atrx, Rnf12, Pdha1, and Pgk1, red) in Eed fl/fl and Eed –/– TSCs. Nuclei are stained blue with DAPI. Scale bar, 2 μm. Right, quantifications of the RNA FISH data. Whereas Atrx, Rnf12, and Pdha1 remain silenced in Eed –/– TSCs, Pgk1 is derepressed from the inactive-X in the absence of EED. Three technical replicates of the Eed fl/fl TSC line and three different Eed –/– TSC lines were stained; 100 nuclei counted/gene/TSC sample