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Table 2 Experimental data used for parameter inference

From: Inferring the physical properties of yeast chromatin through Bayesian analysis of whole nucleus simulations

Dataset

Observable

Experimental technique

Data points (n)

Reference

O1

Median 3D distance between pairs of subtelomeric loci

Imaging, live cells

62

[50]

O2

Median angle between locus, nuclear and nucleolar centroids

Imaging, live cells

37

[31, 50]

O3

Median distance between chromosome 12 locus and nucleolus

Imaging, live cells

15

[64]

O4

Median 2D distance between pairs of loci on chromosome 4

Imaging, live cells

12

this study

O5

Mean 3D distances between intrachromosomal pairs of loci on several chromosomes

Imaging, fixed cells (FISH)

13

[51]

O6

Mode of 3D distances between intrachromosomal pairs of loci on chromosome 14

Imaging, fixed cells (FISH)

8

[80]

O7

Mode of 3D distances between SPB and telomeres

Imaging, fixed cell (Immunofluorescence)

7

[80]

O8

Mean contact frequencies within chromosomes (16) and of each chromosome with the other chromosomes (16) + Intrachromosomal contact frequencies for genomic distances 25–85 Kb at 5 Kb intervals, averaged over the genome (12) + relative to the centromere (12)

Hi-C

56

[29]

O9

Same

Hi-C

56

[30]

 

Total

 

266

 
  1. This table summarizes the nine experimental datasets used for parameter inference in this study. Additional data used for model validation only are: Hi-C data from [62], Micro-C XL data from [63], and chromatin dynamics data from [6466]. For more details, see Additional file 3 and Additional file 1: Supplementary Methods