Fig. 6

Lipidome profiling of dietary restriction (DR)-induced changes in triglyceride (TG) acyl chain length and saturation in the mouse liver. a Hepatic TG content of ad libitum-fed (AL) and DR animals at young and old age. There was a significant interaction between diet and age (two-way ANOVA p < 0.05). b-e Distribution of TG species in young (b, d) and old animals (c, e) classified according to the degree of saturation (b, c) and number of carbon atoms (d, e) as proxy for TG-associated fatty acid chain length. Values represent normalized relative abundances (0–100%) on a logarithmic scale. Indicated are p values for pairwise comparisons for each species (Tukey range test) and for specific intervals (paired Wilcoxon-rank-sum test). DR animals showed significantly more TGs with four or more double bonds at young age (b, p = 0.0039; paired Wilcoxon-rank-sum test) and significantly more TGs with 52 or fewer carbons at old age (e, p = 0.0078; paired Wilcoxon-rank-sum test). AL animals showed significantly more TGs with 54 or fewer carbons at old age (e, p = 0.052; paired Wilcoxon-rank-sum test). • p < 0.1, * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars denote means ± SEM. f, g Schematic outline of differential methylation and gene expression in the Sreb1f (SREBP1) network under AL (f) and DR (g) conditions