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Fig. 6 | Genome Biology

Fig. 6

From: Diverse interventions that extend mouse lifespan suppress shared age-associated epigenetic changes at critical gene regulatory regions

Fig. 6

Age-associated DNA methylation changes are also suppressed by calorie restriction and rapamycin. a Global percentage methylation per sample for 2- (Y) and 22-month-old (O) control, 22-month-old caloric restricted (CR) and 22-month-old rapamycin-treated (Rapa) UM-HET3 mice. Part of this panel is reproduced from Additional file 5: Figure S1c. All p > 0.05 (two tailed t-test on arcsine transformed proportions). b Mean percentage methylation per sample across all O-Y hypomethylated aDMRs for the samples in a. **Y versus O, Y versus Rapa, O versus CR, all p < 0.001; *Y versus CR, O versus Rapa, CR versus Rapa, all p < 0.05 (two tailed t-test on arcsine transformed proportions). c Mean percentage methylation per sample across all O-Y hypermethylated aDMRs for the samples in a. **Y versus O, Y versus Rapa, Y versus CR, all p < 0.001; *O versus CR, CR versus Rapa p < 0.05, O versus Rapa p > 0.05 (two tailed t-test on arcsine transformed proportions). d Clustered feature interaction maps of spatial overlap between O-Y aDMRs (columns) and a selection of genomic, histone and transcription factor features (rows), showing hypomethylated aDMRs that are also CR-O hypermethylated DMRs (i.e. suppressed by CR); 1116 regions. e As d for O-Y hypermethylated aDMRs that are also CR-O hypomethylated DMRs (i.e. suppressed by CR); 359 regions. f As d for O-Y hypomethylated aDMRs that are also Rapa-O hypermethylated DMRs (i.e. suppressed by rapamycin); 330 regions. g As d for O-Y hypermethylated aDMRs that are also Rapa-O hypomethylated DMRs (i.e. suppressed by rapamycin); 225 regions. In dg, the interaction map x-axes are scaled by number of aDMRs. h Composite profiles of mean percentage methylation at all hypomethylated aDMR (Old-Young, UM-HET3 mice) enhancer regions (n = 1867), showing young (blue), old (black), CR (orange) and rapamycin-treated (red). i Composite profiles of mean percentage methylation at hypermethylated aDMR (Old-Young) bivalent regions (n = 536), showing young (blue), old (black), CR (green) and rapamycin-treated (red)

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