Fig. 12

KTI4 influences disease resistance in tomato fruit. a Virus-induced gene silencing (VIGS) assay revealed that KTI4 affects disease resistance in tomato fruit. Immature green stage fruit were infiltrated with an empty vector control pTRV2 (EV) or pTRV2 containing a specific KTI4 sequence (pTRV2-KTI4). Fourteen days after infiltration, the fruit were inoculated with B. cinerea. Disease severity was observed daily after inoculation. b KTI4 gene expression after VIGS, as determined by quantitative RT-PCR. The gene transcript levels were normalized against expression of the ACTIN gene, followed by normalization against the control. Values are shown as the means ± standard deviation. Asterisks indicate significant differences (P < 0.05; t-test) between fruit infiltrated with pTRV2 (EV) and pTRV2-KTI4