TY - JOUR AU - Guillaumet-Adkins, Amy AU - Rodríguez-Esteban, Gustavo AU - Mereu, Elisabetta AU - Mendez-Lago, Maria AU - Jaitin, Diego A. AU - Villanueva, Alberto AU - Vidal, August AU - Martinez-Marti, Alex AU - Felip, Enriqueta AU - Vivancos, Ana AU - Keren-Shaul, Hadas AU - Heath, Simon AU - Gut, Marta AU - Amit, Ido AU - Gut, Ivo AU - Heyn, Holger PY - 2017 DA - 2017/03/01 TI - Single-cell transcriptome conservation in cryopreserved cells and tissues JO - Genome Biology SP - 45 VL - 18 IS - 1 AB - A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs. SN - 1474-760X UR - https://doi.org/10.1186/s13059-017-1171-9 DO - 10.1186/s13059-017-1171-9 ID - Guillaumet-Adkins2017 ER -