Fig. 4

Tissue-specific expression of HYDIN/HYDIN2 isoforms. a RT-PCR analysis over a 15 bp deletion in exon 46 of HYDIN2 compares the relative abundance of HYDIN (top band, 321 bp) and HYDIN2 (bottom band, 306 bp) mRNA in different adult and fetal tissues. Images have been inverted and brightness adjusted for clarity. Adult brain and ovary show the highest levels of HYDIN2 while HYDIN is expressed predominantly in lung, pancreas, and testis. In fetal tissues, HYDIN2 is expressed more ubiquitously. b RNA-seq reads from various tissues (The GTEx Consortium, 2013) containing SUNKs (k = 30) were used to estimate HYDIN and HYDIN2 expression. Full-length HYDIN is expressed most highly in the lung and testis, while all isoforms of HYDIN2 are more highly expressed in brain tissues. Isoform F is not expressed at a level that is likely to be significant. Boxplots indicate median and interquartile range (IQR) with outliers shown beyond 1.5 × IQR