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Table 1 Absolute quantification of exogenous targets

From: RNAs competing for microRNAs mutually influence their fluctuations in a highly non-linear microRNA-dependent manner in single cells

Exogenous transcript

Cotransfection

Cotransfection + pre-miR-20a

eYFP low

58 (42)

41 (28)

eYFP medium

354 (398)

103 (54)

eYFP high

2986 (2840)

336 (28)

mCherry low

34 (18)

10 (9)

mCherry medium

113 (50)

21 (18)

mCherry high

424 (368)

205 (214)

mCerulean low

6 (1)

8 (3)

mCerulean medium

60 (54)

36 (29)

mCerulean high

208 (152)

261 (296)

  1. Absolute quantification of the number of transcripts per cell for the exogenous molecules (standard deviation over the biological replicates in parentheses) for the cotransfection of the two constructs (second column), and the cotransfection of the two constructs plus pre-miR20 (third column). The mean number of mRNA exogenous molecules for three different intervals of eYFP basal expression is low enough to be comparable with physiological values