Skip to main content
Fig. 6 | Genome Biology

Fig. 6

From: RNAs competing for microRNAs mutually influence their fluctuations in a highly non-linear microRNA-dependent manner in single cells

Fig. 6

Interplay between transcriptional activity and miRNA–target interaction strength. The figure shows model predictions and experimental results obtained when investigating the effect on one target (say p 1) of the interplay between the second target (say r 2 and, thus, p 2) and the miRNA. The interplay between r 2 and miRNA is tuned both via the transcription rate \(k_{r_{2}}\) of r 2 and via the interaction strength g 2 between r 2 and the miRNA. p 1 is plotted against p 0 on a increasing the transcription rate \(k_{r_{2}}\) of r 2, b increasing the interaction strength g 2 between miRNA and r 2 when \(k_{r_{2}} > k_{s}\) (excess of targets), and c increasing the interaction strength g 2 between miRNA and r 2 when \(k_{r_{2}} < k_{s}\) (excess of miRNA). The model prediction for cases depicted in (a) and (b) are qualitatively very similar. d mCherry mean fluorescence (a proxy for p 1 in the model) is plotted against eYFP (a proxy for the constitutive expression p 0 in the model). The dashed black line corresponds to the unregulated case while the blue data points correspond to the reference case with four MREs on mCherry and one MRE on mCerulean. Either increasing the copy number of mCerulean (a proxy for \(k_{r_{2}}\) in the model), black data points, or the number of MREs on its sequence (a proxy for g 2 in the model), red data points, has the effect of decreasing the amount of miRNA available to target mCherry (which globally increases). e Fold-repression with respect to the unregulated case plotted against eYFP. Error bars are evaluated on the biological replicates. a.u. arbitrary units, eYFP enhanced yellow fluorescent protein, miRNA microRNA, MRE miRNA regulatory element

Back to article page