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Fig. 7 | Genome Biology

Fig. 7

From: RNF40 regulates gene expression in an epigenetic context-dependent manner

Fig. 7

The Hoxc gene cluster as a prototype for RNF40/H2Bub1-dependent gene regulatory mechanisms. a The Hoxc gene cluster displays an enrichment of repressive modifications (H3K27me3) and low mRNA expression at the 5’ portion of the locus and active modifications (H3K4me3, H3K27ac) and higher mRNA levels at the 3' portion of the locus. b ChIP-qPCR analyses of RNA Polymerase II occupancy near the TSS of the Hoxc13 and Hoxc6 genes in the three samples. The data are shown as mean ± SD (n = 3). *p < 0.05; **p < 0.001; n.s. non-significant; unpaired two-tailed t-test. c qRT-PCR analysis of Hoxc13 and Hoxc6 in Rnf40 +/+, Rnf40 –/–, and EZH2i-treated Rnf40 +/+ samples (examined in 4D). Mean ± SD, n = 3; * p < 0.05; ** p < 0.001; n.s. non-significant; unpaired two-tailed t-test. dg qRT-PCR analysis of the expression of the RASD1, PPARG, HOXB2, and HOXC10 genes in control and adipocyte-differentiated hMSC transfected with non-targeting siRNA (sicon) and RNF40 siRNAs (siRNF40). qPCR was performed using the same samples examined in Additional file 1: Figure S5F

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