Fig. 1
From: Genome-wide mapping of 5-hydroxymethyluracil in the eukaryote parasite Leishmania
The 5hmU enrichment strategies. a 5-Hydroxymethyluridine (5hmU, left), 5-formyluridine (5fU, middle), and chemically tagged 5fU. b 5hmU chemical pull-down. MeO 5′-O-methyl, p 3′-phosphate. c LC-MS traces of 5fU-modified 10mer ODN (ODN-5fU) after biotinylation (top), followed by treatment in 20 mM phosphate buffer (pH 6) supplemented with p-anisidine (center) or 20 mM phosphate buffer (pH 6) supplemented with p-anisidine and NH2OH (bottom). Observed mass signals in peaks A and B (right panel)