Skip to main content
Fig. 2 | Genome Biology

Fig. 2

From: Discovery and functional prioritization of Parkinson’s disease candidate genes from large-scale whole exome sequencing

Fig. 2

High-content assay for mitochondrial morphology. Effect of DNM1L shRNA (a, b) and UHRF1BP1L shRNA (c, d). BE(2)M17 cells stained with Hoechst (blue; nuclei), MitoTracker CMXros, and MitoTracker Deepred (yellow; mitochondria). a Cells infected with shRNA encoding a scrambled sequence (SCR, left panel) and decrease in mitochondrial axial length ratio and roundness for DNM1L (positive control, right panel). b The graph displays normalized mitochondrial roundness. c Cells infected with shRNA encoding a SCR sequence (left panel) and decrease in number of mitochondria per cell, mitochondrial axial length ratio, and roundness for UHRF1BP1L (right panel). d The graph displays normalized mitochondrial roundness. Data are median values ± median absolute deviation (MAD) of N = 6 measurements. *p < 0.05 and **p < 0.01, Mann–Whitney U test (see “Methods”). All values were normalized to the negative control (infected with SCR shRNA) and all shRNA clones that meet the cutoff criteria are shown (b, d)

Back to article page

Genome Biology

ISSN: 1474-760X

Contact us