Table 1 Summary of nuclear transfer results from gene-targeted bovine fetal fibroblast cells
From: Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects
Nuclear donor | Cas9 nuclease | Cas9 nickase | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Typical colonies suitable for SCNT | Total | Typical colonies suitable for SCNT | Total | |||||||
Cell clone | 45–57 | 45–86 | 45–136 | 45–159 | - | 45–113 | 45–187 | 45–243 | 45–351 | - |
Embryos obtained | 632 | 578 | 644 | 531 | 2385 | 568 | 674 | 527 | 665 | 2434 |
Blastocysts (%) | 137 (21.7) | 143 (24.7) | 126 (19.6) | 119 (22.4) | 525 (22.0) | 163 (28.7) | 210 (31.2) | 157 (29.8) | 223 (33.5) | 753 (30.9) |
Recipients | 46 | 49 | 41 | 37 | 173 | 54 | 68 | 51 | 75 | 248 |
Pregnancies (%) | 6 (13.0) | 9 (18.4) | 2 (4.9) | 5 (13.5) | 22 (12.7) | 9 (16.7) | 18 (26.5) | 11 (21.6) | 23 (30.7) | 61 (24.6) |
Calves at birth | 1 | 2 | 0 | 1 | 4 | 2 | 5 | 3 | 6 | 16 |
Survived for 3Â months | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 2 | 3 | 9 |