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Table 1 Summary of nuclear transfer results from gene-targeted bovine fetal fibroblast cells

From: Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

Nuclear donor Cas9 nuclease Cas9 nickase
Typical colonies suitable for SCNT Total Typical colonies suitable for SCNT Total
Cell clone 45–57 45–86 45–136 45–159 - 45–113 45–187 45–243 45–351 -
Embryos obtained 632 578 644 531 2385 568 674 527 665 2434
Blastocysts (%) 137 (21.7) 143 (24.7) 126 (19.6) 119 (22.4) 525 (22.0) 163 (28.7) 210 (31.2) 157 (29.8) 223 (33.5) 753 (30.9)
Recipients 46 49 41 37 173 54 68 51 75 248
Pregnancies (%) 6 (13.0) 9 (18.4) 2 (4.9) 5 (13.5) 22 (12.7) 9 (16.7) 18 (26.5) 11 (21.6) 23 (30.7) 61 (24.6)
Calves at birth 1 2 0 1 4 2 5 3 6 16
Survived for 3 months 0 1 0 1 2 1 3 2 3 9