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Fig. 6 | Genome Biology

Fig. 6

From: Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

Fig. 6

Assessment of the transgenic cattle. a Transgenic colony after positive drug selection and transgenic embryos from SCNT under a fluorescence stereomicroscope. b Photographs of one-month-old calves that carried the NRAMP1 insertion. c The 5′ (left, 1.58 kb) and 3′ junction (right, 1.98 kb) PCR analyses confirming the site-specific targeting in the transgenic cattle. “Con” represents the control normal cattle. The templates for PCR were genomic DNA samples that were extracted from the peripheral blood of cattle. d Southern blot analysis of the genomic DNA extracted from transgenic cattle. “N” represents the negative control normal cattle; “P” represents the positive transgenic BFFs

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