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Table 1 Distribution of methylated Cs in transcripts of total and nuclear poly(A) RNA of ESCs and brain

From: Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain

 

Fisher exact test

No. of m5Cs tested

p value*

Odds ratio

95% confidence interval

Total poly(A) RNA

 ESC

  5′ UTR

1.84E-37

1.74

1.60–1.88

714

  CDS

2.22E-08

0.86

0.81–0.90

2828

  3′ UTR

0.033

0.94

0.89–0.99

1775

  AUG (+/– 25 nt)

2.87E-29

2.38

2.07–2.72

225

 Brain

  5′ UTR

1.02E-81

3.51

3.13–3.94

389

  CDS

3.11E-106

0.31

0.28–0.35

399

  3′ UTR

6.02E-19

1.55

1.41–1.71

863

  AUG (+/– 25 nt)

6.93E-27

3.84

3.09–4.71

98

Nuclear poly(A) RNA

 ESC

  5′ UTR

2.49E-09

1.36

1.23–1.50

459

  CDS

3.53E-17

0.78

0.73–0.82

2337

  3′ UTR

1.31E-07

1.18

1.10–1.25

1688

  AUG (+/– 25 nt)

1.75E-30

2.57

2.19–2.96

203

 Brain

  5′ UTR

0.001

1.37

1.13–1.65

123

  CDS

1.74E-244

0.11

0.09–0.13

157

  3′ UTR

1.12E-208

6.23

5.51–7.21

1028

  AUG (+/– 25 nt)

1.98E-19

3.90

3.00–4.99

67

  1. *Significance threshold p < 0.05